1
Eshly isolated AMs from MS-/- mice, the cell lines exhibit decreased phagocytosis of unopsonized titanium dioxide (TiO2), fluorescent latex beads and bacteria (Staphylococcus aureus) compared with the primary AMs from wild type (WT) C57BL/6 mice. Conclusion: Our results indicated that three contiguous murine alveolar macrophage cell lines with MS-/- (ZK1, ZK2 and ZK6) were established successfully
1
Ing time (mean generation time = mgt) was calculated according the formula: N = N02T/mgt. On the average, doubling time of ZK cell lines is between 12 h to 16 h in RPMI complete medium (Fig. 2). Like their parental primary AMs isolated from the MS-/- mice, all of the cell lines are adherent but trypsin-sensitive for passage. Morphology Light microscopic examination of Diff Quik, a modified Wright-
1
Further define MARCO and SR-AI/II function, and may also be useful to identify other novel scavenger-type macrophage receptors and for additional studies of particle toxicology.BackgroundThe pulmonary alveolar macrophage (AM) plays an important role in defense of the lung [1-5]. Class A scavenger receptors (SRA) primarily expressed on the macro-phage (M? surface are critical for binding, uptake, a
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R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function
1
Mpled sequences are likely CRF02_AG (accounting for 50 of HIV-1M infections), with the other "pure" subtypes (G, D, A, and F) and CRFs (CRF11_cpx, 36_cpx, 37_cpx, and CRF01_AE) accounting for the remainder of infections. CRF02_AG and clade G viruses are broadly distributed across west central Africa and have apparently been circulating stably there for many years [3,17-19], consistent with the pr
1
Dian overall survival (Figure 3). CISH analysis was possible in 44 cases.Survival probability ( )0 0 5 10 15 Months 20 25FISH EGFR = 0.2) of colorectal FISH patients showing Overall 3 (pGCN 2.6 (-------) andcancerEGFR GCN
1
Eshly isolated AMs from MS-/- mice, the cell lines exhibit decreased phagocytosis of unopsonized titanium dioxide (TiO2), fluorescent latex beads and bacteria (Staphylococcus aureus) compared with the primary AMs from wild type (WT) C57BL/6 mice. Conclusion: Our results indicated that three contiguous murine alveolar macrophage cell lines with MS-/- (ZK1, ZK2 and ZK6) were established successfully
1
Nowledgements The authors are grateful to Andile Nofemela and Roman Ntale for technical assistance with viral sequencing. This research was supported by the International Atomic Energy Agency (technical co-operation project RAF/6/ 029), Poliomyelitis Research Foundation (PRF) of South Africa and the University of Cape Town, for collaborative projects with partners in the global South. We thank Ger

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