1
Tential impact of HIV-1 diversity on both vaccine development and the sustainability of antiretroviral therapies, it is particularly important that molecular epidemiological surveillance is continued in HIV diversity hotspots such as Cameroon. In this study we have focused on characterizing the diversity of gag and nef genes of Cameroonian HIV-1 isolates. These genes are?2013 Tongo et al.; license
1
Ormation were obtained. RNA was extracted from plasma samples, reverse transcribed and PCR amplified as described previously [12] using subtype non-specific HIV-1 primers for HIV-1 full length gag [12] and nef [13] genes, and sequenced. Sequenced fragments were assembled using ChromasPro. Full length gag and nef sequences were generated and aligned using MUSCLE with manual editing in MEGA5, togeth
1
Onfim I, Camacho RJ, Vandamme AM, Lemey P: Phylodynamics of the HIV-1 CRF02_AG clade in Cameroon. Infect Genet Evol 2012, 12:453?60. 20. Zhang M, Foley B, Schultz AK, Macke JP, Bulla I, Stanke M, Morgenstern B, Korber B, Leitner T: The role of recombination in the emergence of a complex and dynamic HIV epidemic. Retrovirology 2010, 7:25. 21. Carr JK, Salminen MO, Albert J, Sanders-Buell E, Gotte D
1
Nowledgements The authors are grateful to Andile Nofemela and Roman Ntale for technical assistance with viral sequencing. This research was supported by the International Atomic Energy Agency (technical co-operation project RAF/6/ 029), Poliomyelitis Research Foundation (PRF) of South Africa and the University of Cape Town, for collaborative projects with partners in the global South. We thank Ger
1
A CRF02_AGa CRF02_AGa CRF02_AGaCRF02_AG CRF22_01A1 CRF02_AG CRF02_AG CRF36_cpxb/F2b CRF02_AG NDc CRF02_AG CRF02_AG ND NDc cCRF02_AG CRF01_AE CRF02_AG CRF02_AG CRF01/F CRF02_AG A-likeb CRF02_AG CRF02_AG CRF01_AE CRF02_AG NDc CRF02_AGNDc CRF02_AG A1 A1 F G A1 CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF37_cpx F CRF01_AE CRF37_cpx Ub DCRF02_AGa CRF02_AG URF A1 URF G URF
1
Hose residing on isolated branches outside of subtrees containing previously defined HIV-1 subtype or CRF lineages. Outlier sequences on the other hand were defined as those residing on basal branches of subtrees containing previously defined HIV-1 subtype or CRF lineages. Nucleotide sequences were deposited in GenBank [JX244899-JX244948 for gag and JX244949JX245003 for nef]. Clinical and demograp
1
A CRF02_AGa CRF02_AGa CRF02_AGaCRF02_AG CRF22_01A1 CRF02_AG CRF02_AG CRF36_cpxb/F2b CRF02_AG NDc CRF02_AG CRF02_AG ND NDc cCRF02_AG CRF01_AE CRF02_AG CRF02_AG CRF01/F CRF02_AG A-likeb CRF02_AG CRF02_AG CRF01_AE CRF02_AG NDc CRF02_AGNDc CRF02_AG A1 A1 F G A1 CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF02_AG CRF37_cpx F CRF01_AE CRF37_cpx Ub DCRF02_AGa CRF02_AG URF A1 URF G URF
1
R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function

Social Bookmarking Sites
What is Kliqqi?

Kliqqi is an open source content management system that lets you easily create your own user-powered website.

Latest Comments

(function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){ (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o), m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m) })(window,document,'script','https://www.google-analytics.com/analytics.js','ga'); ga('create', 'UA-98700619-1', 'auto'); ga('send', 'pageview');